PCR plate are essential tools in molecular biology, and researchers use them for various applications such as genetic analysis, diagnostics, and research. PCR plate contain multiple wells, with each well holding a sample or reaction mixture for the Polymerase Chain Reaction (PCR). Proper storage of PCR plate is crucial for ensuring the accuracy and reliability of your experiments. In this blog post, we will explore how long you can store PCR plates, the factors that influence their shelf life, and the best practices for maintaining their integrity.
Factors That Affect How Long PCR Plate Can Be Stored
Type of PCR Plate
The material and construction of the PCR plate can affect its storage longevity. PCR plates are typically made from polypropylene or polystyrene. Polypropylene plates are more resistant to temperature fluctuations and chemical interactions, making them suitable for longer storage periods. On the other hand, polystyrene plates may be less durable and not suitable for extended storage.
Contents of the PCR Plate:
The type of samples and reagents in the PCR plate can significantly influence its storage duration. Plates containing DNA, RNA, or other biological materials need specific conditions to prevent degradation. Reagents like enzymes and buffers also have their own shelf lives, and their effectiveness can diminish over time.
Storage Conditions:
Conditions under which PCR plates are stored play a crucial role in determining their shelf life. Key factors include:
- Temperature: PCR plates should be stored at temperatures that prevent sample and reagent degradation. For long-term storage, freezing at -20°C or -80°C is recommended. Short-term storage might be done at 4°C, but this depends on the stability of the reagents and samples.
- Light Exposure: Protect PCR plates from direct light, which can cause degradation of light-sensitive components. Store them in opaque containers or in dark storage areas.
- Humidity: High humidity can affect the quality of PCR plates if they are not sealed properly. Store plates in dry conditions to avoid moisture-related issues.
Sealing and Packaging:
Best Practices for Storing PCR Plate
- Remember to clearly label PCR plates with essential information such as the date of preparation, contents, and any special handling instructions. This will help in tracking and organizing samples and reduce the risk of mix-ups.
- To minimize the impact of frequent freeze-thaw cycles on samples and reagents, aliquot them into smaller volumes if possible, and avoid repeatedly thawing and refreezing the entire plate.
- Regularly check the storage conditions, including temperature and humidity, to ensure they remain within recommended ranges. Using temperature monitoring devices can assist in maintaining optimal storage conditions.
- Invest in high-quality sealing materials, such as adhesive films or lids, to protect PCR plates from contamination and environmental factors. Make sure the seals are properly applied and intact.
- Handle PCR plates gently to avoid physical damage. Use clean gloves and avoid touching the wells or surfaces directly to prevent contamination.